Barnsley lost 32-16 home to leaders Halifax in Yorkshire Three.
The Shaw Laners are now in the relegation zone after slipping down a place this week.
They have lost five out of six games so far this season.
Barnsley are now due to visit Leeds Corinthians who are third.
Barnsley Ladies lost 35-7 at Sale in Championship One North.
Wath suffered a second successive loss as they went down 38-14 at home to Pocklington in Yorkshire Division One.
Oscar Bramhall scored a try, converted by Jack Whitlam, with another penalty try.
Wath are sixth after six games, with four wins and two losses.
They now visit third-bottom Bridlington.
Dearne Valley’s scheduled visitors Knottingley pulled out in Yorkshire Division Four.
Dearne were given five points so stay top.
They are due to visit Sheffield Medicals on Saturday.
Wortley lost 29-20 to Hessle.
They are now due to visit Knottingley.
