Barnsley will travel to Manchester United on Tuesday September 17 for an 8pm kick-off in their EFL Cup third round game.
The Reds have been given an allocation of 7,393 and adult prices are £41/42.
Barnsley earned the draw at the Premier League giants with wins over Sheffield United and Wigan Athletic in the first two rounds.
It will be their first meeting with United since a 2-0 loss in 2009 at Oakwell in the same competition.